1.
Indian J Biochem Biophys
;
1989 Jun; 26(3): 131-5
Article
in English
| IMSEAR
| ID: sea-27610
ABSTRACT
Nuclear DNA isolated from hypocotyls (H), proliferating callus (PC) and differentiating callus (DC) of Brassica juncea contains a satellite DNA which can be resolved in actinomycin-D/CsCl gradients. The satellite DNA undergoes changes, when an in vitro culture is raised from hypocotyl tissue and forms a higher percentage of the genome in PC and DC than in mature differentiated tissue (hypocotyl). All the three satellite DNAs are GC-rich compared to main band DNAs. Satellite DNA of H has higher Tm and GC content than that of the PC and DC satellites. A 200 bp basic repeat unit from hypocotyl nuclear DNA has been cloned and characterised.
Subject(s)
Brassica/cytology , Cell Differentiation/genetics , Cell Division/genetics , Culture Techniques , DNA, Satellite/isolation & purification
2.
Indian J Biochem Biophys
;
1982 Aug; 19(4): 278-9
Article
in English
| IMSEAR
| ID: sea-27390